张雅婕, 林华, 周少磊, 田竞, 贾予平, 曹文婧, 何立伟, 姚晓慧, 刘晋源, 高斌, 张永, 沈凡. 脂多糖生物探针法与传统培养法检测水中嗜肺军团菌的比较[J]. 环境卫生学杂志, 2024, 14(9): 788-792. DOI: 10.13421/j.cnki.hjwsxzz.2024.09.011
    引用本文: 张雅婕, 林华, 周少磊, 田竞, 贾予平, 曹文婧, 何立伟, 姚晓慧, 刘晋源, 高斌, 张永, 沈凡. 脂多糖生物探针法与传统培养法检测水中嗜肺军团菌的比较[J]. 环境卫生学杂志, 2024, 14(9): 788-792. DOI: 10.13421/j.cnki.hjwsxzz.2024.09.011
    ZHANG Ya-jie, LIN Hua, ZHOU Shao-lei, TIAN Jing, JIA Yu-ping, CAO Wen-jing, HE Li-wei, YAO Xiao-hui, LIU Jin-yuan, GAO Bin, ZHANG Yong, SHEN Fan. Comparison of lipopolysaccharide bioprobe method and traditional culture methods for quantifying Legionella pneumophila in water[J]. Journal of Environmental Hygiene, 2024, 14(9): 788-792. DOI: 10.13421/j.cnki.hjwsxzz.2024.09.011
    Citation: ZHANG Ya-jie, LIN Hua, ZHOU Shao-lei, TIAN Jing, JIA Yu-ping, CAO Wen-jing, HE Li-wei, YAO Xiao-hui, LIU Jin-yuan, GAO Bin, ZHANG Yong, SHEN Fan. Comparison of lipopolysaccharide bioprobe method and traditional culture methods for quantifying Legionella pneumophila in water[J]. Journal of Environmental Hygiene, 2024, 14(9): 788-792. DOI: 10.13421/j.cnki.hjwsxzz.2024.09.011

    脂多糖生物探针法与传统培养法检测水中嗜肺军团菌的比较

    Comparison of lipopolysaccharide bioprobe method and traditional culture methods for quantifying Legionella pneumophila in water

    • 摘要:
      目的 比较脂多糖生物探针法和传统培养法(包括ISO 11731-2017法即“ISO法”、GB/T 18204.5-2013法即“国标法”)对水中嗜肺军团菌(Legionella pneumophila)的检测能力。
      方法 分别采用脂多糖生物探针法和传统培养法对58份加标水样(其中50份为实际水样加标用于以上两类方法比对测试, 8份为纯水加标用于脂多糖生物探针法的方法学性能测试)和50份实际水样进行检测, 确定脂多糖生物探针法的检出限、准确度、精密度、特异性和灵敏度, 并比较两类方法对50份实际水样加标前的定性结果和50份实际水样加标前后的定量结果。
      结果 脂多糖生物探针法检出限为1.55 CFU/L, 准确度为102.10%~139.29%, 精密度为0.14%~1.78%, 特异性为95.12%, 灵敏度为88.89%。50份实际水样加标前脂多糖生物探针法和国标法两种方法(检出或未检出)的定性结果无统计学差异(P > 0.05);50份实际水样加标前后脂多糖生物探针法定量结果的中位数(M)高于ISO法测定结果, 差异具有统计学差异(P < 0.001), 两类方法测定结果存在相关(r = 0.981)。脂多糖生物探针法检出限(1.55 CFU/L)低于ISO法的(15.74 CFU/L), 检测时间(2 d)短于ISO法(10 d)。
      结论 与传统培养法相比, 脂多糖生物探针法耗时短, 检测性能更优。

       

      Abstract:
      Objective To compare the detection capabilities of the lipopolysaccharide bioprobe method and traditional culture methods (including ISO 11731-2017 and GB/T 18204.5-2013) for quantifying Legionella pneumophila in water.
      Methods The lipopolysaccharide bioprobe method and traditional culture methods were applied separately to test 58 spiked water samples (50 spiked actual water samples for method comparison and 8 spiked purified water samples for performance test of the lipopolysaccharide bioprobe method) and 50 actual water samples. The detection limit, accuracy, precision, specificity, and sensitivity of the lipopolysaccharide bioprobe method were determined. The methods were compared for the qualitative results of 50 actual water samples before spiking and the quantitative results of 50 actual water samples before and after spiking.
      Results For the lipopolysaccharide bioprobe method, the detection limit was 1.55 CFU/L, the accuracy was 102.10%-139.29%, the precision was 0.14%-1.78%, the specificity was 95.12%, and the sensitivity was 88.89%. There was no significant difference in the qualitative results (detected or undetected) of 50 actual water samples before spiking between the lipopolysaccharide bioprobe method and the GB/T method (P>0.05). For 50 actual water samples before and after spiking, the medians (M) of the quantitative results with the lipopolysaccharide bioprobe method were significantly higher than those with the ISO method (P < 0.001), and the results with the two method were significantly correlated with each other (r=0.981). The detection limit of the lipopolysaccharide bioprobe method (1.55 CFU/L) was lower than that of the ISO method (15.74 CFU/L), and the detection time of the former (2 d) was shorter than that of the latter (10 d).
      Conclusion The lipopolysaccharide bioprobe method is a new method for detecting Legionella pneumophila in water, with shorter detection time and better detection performance compared with traditional culture methods.

       

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